5 Tips about attune flow cytometer You Can Use Today

Data-enriched characterization of mobile populations. Morphological details from photos can include into the richness of flow cytometry facts. By way of example, the determine shows an in any other case common apoptosis assay utilizing Annexin V and PI, incorporating cell imaging to characterize cells in Each and every population to expose morphologically distinctive features. These insights could not are actually attained from multiplex staining on your own.

As samples are acquired about the Attune CytPix Flow Cytometer, the superior-pace brightfield camera captures and stores pictures of detected functions at a charge around six,000 photos for each second, according to the flow level and image dimension. For larger flexibility, Attune Cytometric Software program allows you to alter the graphic seize frequency as needed and select a particular gate for impression seize, if needed.

Blend acoustic focusing technology with large-parameter Investigation for versatile spectral unmixing and traditional workflows.

Find out new insights into your experimental knowledge by visualizing populations utilizing combined fluorescence and morphology info. Our program interface wizards allow downsampling, concatenation (merged documents) and dimensionality resources which are developed into the software for an intuitive, streamlined facts analysis encounter.

*Pricing is indicative only and is matter to vary. You should Speak to your Agilent agent with any inquiries. The information established forth herein isn't a proposal or even a determination, each of that are subject matter to credit rating acceptance, conditions and terms, and availability with Agilent. Agilent Economic Options is available in picked nations.

Below, a skilled consumer has gated singlets confidently. Immediately after evaluating the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of a lot more than four% aggregates.

Optimizing gating approach. Even sturdy guide singlet gating is error-inclined and continues to be a subjective final decision issue in Pretty much all flow cytometry assays. Imaging can be used to verify and alter gates to include only single cells of fascination.

Regular image click here top quality even at higher throughput Acoustic concentrating in addition to a significant-pace digital camera Mix to image these T cells continuously at low or superior flow premiums. Simply adjust aim and digital camera configurations to fulfill experimental demands.

Early indications of this contamination experienced at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also permits you to use morphological features to find out intriguing subpopulations that would not be apparent from flow cytometry info alone.

Customers can perspective occasion images in actual-time to verify sample high quality, enhance protocols, and record the morphology of every mobile population discovered for analysis. Imaging capture also benefits from acoustic concentrating, which helps you to posture the cells to ensure a pointy, centered picture is acquired.

AI-driven automated picture Evaluation While using the Attune Cytometric Software interprets celebration attributes into distinct morphology parameters that could be coupled with standard fluorescence and scatter parameters to expose highly reproducible population variations and special subsets.

Software note: Automatic image Investigation minimizes user-to-person variability in flow cytometry gating approaches

Created-in dimensionality reduction equipment enhanced resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify anticipated inhabitants resolution in lysed whole human blood. Consumer can look at functions from each cluster for visual confirmation of mobile phenotypes.

Adaptable payment strategy from Agilent Fiscal Remedies help you have the essential instrumentation you require for instant challenge, or to mature your lab.

At the guts of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological know-how, which utilizes seem waves to meticulously align cells throughout the flow cell. This progressive technique surpasses regular hydrodynamic methods, leading to more quickly processing speeds, Excellent knowledge excellent, and amplified sample throughput.

The Attune Xenith cytometer is often a superior-parameter spectral analyzer, offering both equally spectral unmixing and conventional flow capabilities for in depth and versatile mobile Assessment.

The Attune CytPix Flow Cytometer enables customers to visualise samples through acquisition, validate the precision of gating strategies, and guidance effects with trusted documentation for publication purposes.

Right here, we display that by gating procedures utilizing picture-centered quantitative parameters can distinguish interacting cells from coincident occasions to far more correctly review interacting cells.